FGL Notes

Protocols

Pre-Printing Protocols

Print Plate Resuspension    Print Plate Resuspension   last modified by Vivian Peng on 1/20/2004

Poly-l-lysine Coating Protocol    Poly-l-lysine slide Coating Protocol    submitted by Vivian Peng on 12/15/2003

Library Production

Colony PCR    Colony PCR from an pre-arrayed library set    submitted by Vivian Peng on 12/15/2003

Consolidation of print plates    Consolidation of print plates    submitted by Vivian Peng on 12/15/2003

PCR Gels    PCR Gels    submitted by Vivian Peng on 12/15/2003

PPT Gels    PPT Gels    submitted by Vivian Peng on 12/15/2003

Precipitation of 96 well PCR    Precipitation of 96 well PCR    submitted by Vivian Peng on 12/15/2003

ReArray Protocol    ReArray Protocol    submitted by Vivian Peng on 12/15/2003

RNA Aquisition

RNA Isolations    RNA Isolations    submitted by Vivian Peng on 12/15/2003

Small Scale RNA Isolations        submitted by Vivian Peng on 4/25/2007

Modified RNA Small Sample Prep        submitted by Vivian Peng on 6/4/2007

Oligo Array Protocols

Oligo Total RNA Labeling        submitted by Vivian Peng on 5/30/2006

Oligo Array Blocking Protocol        submitted by Vivian Peng on 5/30/2006

Oligo Post Hyb Wash       last modified by Vivian Peng on 6/1/2006

cDNA array Protocols

Amplification    Single round amplification    submitted by Vivian Peng on 10/28/2003

Total RNA Labelling Protocol    Total RNA Labelling Protocol    submitted by Vivian Peng on 12/1/2003

Post Hybridization Washes    Post Hybridization Washes    submitted by Vivian Peng on 12/1/2003

Slide Coating    Slide Coating    submitted by Vivian Peng on 12/1/2003

Post Processing (Blocking) cDNA Arrays    Post Processing (Blocking) cDNA Arrays    submitted by Vivian Peng on 12/1/2003

Materials List

Materials List

Materials List    Materials List   last modified by Vivian Peng on 1/20/2004

array lists / gal files

zfish gals

Zfish P1.1 6/19 - 7/2/2003    gal file for Zfish P1.1    submitted by Vivian Peng on 1/20/2004